Composite

Part:BBa_K5507010:Design

Designed by: josiazhang josiazhang   Group: iGEM24_HW-ShanghaiUnited   (2024-09-29)


gdh-5-Ptac-kana-rrnB-T-gdh-3


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1886


Design Notes

The gdh gene in K. xylinus encodes glucose dehydrogenase, an enzyme that catalyzes the glucose to gluconate. The production of gluconate will reduce the amount of glucose in the medium and affect the production of bacteria cellulose (BC). Comparative studies have been carried out and some isolates that are more capable of producing cellulose from glucose have been found to have lower GDH activity. Therefore, we will knock out the gdh gene to achieve an increase in BC yield.


Source

The part is Composite parts.

References